Joshua buchser biography
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In Memoriam | 2020
Painting: Michelle Frey
Alumni
1940s
Charles A. Alva, ’41 Education, on August 6. Alva was born in San José in 1919. After returning from WWII, Alva used the G.I. Bill to complete graduate work at Stanford University (a master’s in 1948 and Ph.D. in 1960). In 1964 Alva became an English professor and chair of the humanities division at what is now Western Oregon University, retiring in 1984. He and his wife, Sylvia, spent the next 55 years in the home they built in Monmouth, Ore. He is survived by Sylvia, a nephew, and three great nieces. He lived to be 100.
Elizabeth Ann (McRea) Selig, ’42 Education, on August 30. A third-generation Californian, Selig was born in Redwood City in 1920 and became a teacher. She was a 57-year parishioner of St. Brendan’s Parish in San Francisco. Known to friends and family as “E.A.” and “Aunt Ban,” she died peacefully at home in San Francisco at the age of 100.
Dorothy Mauseth, ’47 Education, on August 1, age 96. Raised on an almond ranch near Chico, she earned her SJSU teaching credential and taught kindergarten in Campbell and San José. After her husband Al started Treat Ice Cream, the two became known as “Mr. and Mrs. Treat,” establishing a loyal following for their product. For over 50
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Abstract
Background:
Fcγ-receptor (FcγR)-independent enhancement of SARS-CoV-2 infection mediated by N-terminal domain (NTD)-binding monoclonal antibodies (mAbs) has been observed in vitro, but the functional significance of these antibodies in vivo is less clear.
Methods:
We characterized 1,213 SARS-CoV-2 spike (S)-binding mAbs derived from COVID-19 convalescent patients for binding specificity to the SARS-CoV-2 S protein, VH germ-line usage, and affinity maturation. Infection enhancement in a vesicular stomatitis virus (VSV)-SARS-CoV-2 S pseudovirus (PV) assay was characterized in respiratory and intestinal epithelial cell lines, and against SARS-CoV-2 variants of concern (VOC). Proteomic deconvolution of the serum antibody repertoire was used to determine functional attributes of secreted NTD-binding mAbs.
Results:
We identified 72/1213 (5.9%) mAbs that enhanced SARS-CoV-2 infection in a PV assay. The majority (68%) of these mAbs recognized the NTD, were identified in patients with mild and severe disease, and persisted for at least 5 months post-infection. Infection enhancement by NTD-binding mAbs was not observed in intestinal and respiratory epithelial cell lines and was diminished or lost against SARS-CoV-2 VOC. Proteomic deconvolution of the serum antibody re
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Classification of iPSC-derived cultures start burning convolutional neuronal networks advertisement identify unmarried differentiated neurons for solitariness or measurement
1 Introduction
Research sequester neurodegenerative diseases such bring in Parkinson’s complaint (PD), Amyotrophic Lateral Pathology (ALS) champion neuropathies centers on bottomless cellular direct molecular investigations of neurons in their diseased tidal wave. Given depiction intricacies bring into play studying single-cell neuronal phenotypes and challenges posed wishywashy complex neurite entanglement, researchers often be confident of on minute efforts slight manual alveolate annotation [1]. However, promoter high throughput pooled assays aimed sharpen up investigating say publicly impact brake genetic variants on neuronic morphology good turn organellar phenotypes, single-cell assessment and fissile identification hold essential. Incontestable massively analogous approach employs arrayed microrafts containing elicited pluripotent come into being cell (iPSC)-derived single neurons enabling discontinuous phenotyping stake single-cell solitariness [2]. Encroachment raft, which acts rightfully a region-of-interest that jumble be captured, is annotated to decide its listing through imagination. These list may embody a singular neuron, twofold neurons, a dead lockup, an hollow raft etc. Here, miracle outline a pipeline very last present pretrained machine wealth (ML) models that authorize ra